We have developed a highly efficient Protein profiling method by combining High Performance Liquid Chromatography (HPLC) method of protein separation with ultrasensitive laser induced fluorescence detection. The proteins are detected by their intrinsic fluorescence, on excitation with 257 nm radiation. The efficacious studies with the High Performance Liquid Chromatography- Laser Induced Fluorescence (HPLCLIF) system has made possible the early detection of enzymes and surface molecules associated with tumour cells in all types of malignancy. The studies also include analyses of body fluids to detect biological markers in any kind of disease. HPLC-LIF protein profiling can be used with high sensitivity and specificity for the discrimination of clinical samples of normal and malignant subjects.