Manipal > Institutions > Life Sciences > MLSC Manipal > Research > Core area of research > Biophysics Unit


Select a Course 

  1. Identification and characterization of tumour markers in oral cancer by HPLC-LIF and mass spectroscopy
    In this study, HPLC-LIF will be used to detect tumour markers of oral cancer at much lower concentrations. The tumour fractions identified as responsible for the cancer will be collected and mass spectrometric study will be carried out to determine their masses.

  2. Development of Laser-Induced Fluorescence (LIF) and Photoacoustic Spectroscopy (PAS) instrumentations for cancer diagnosis
    We have designed and developed experimental set-ups for recording LIF and PAS signatures of various cancerous and non-cancerous tissues in vitro. Classification analysis has been carried out using Principal Component Analysis (PCA), Artificial Neural Network (ANN) and K- Nearest Neighbor (KNN) algorithms. The main aim of this program was to detect cancers early at the pre-malignant level and our attempts in this line are quite positive.
  3. Study of dynamics of Biomolecules
    In this study, dynamical nature of biomolecules is studied using simultaneous pulsed laser induced fluorescence and photoacoustic measurements of biomolecules. The techniques, fluorescence and photoacoustic spectroscopy are complementary in nature and measuring these signatures gives information about the dynamics of the biomolecules.
  4. Development of fibre optic probe based low level laser therapy (LLLT) for tissue regeneration
    The proposed project aims to develop a fibre optic probe-based low-level laser therapy for enhanced tissue regeneration. There are few attempts on the utilisation of laser for enhanced tissue regeneration; the proper utilisation of laser power for optimal results is of immediate interest. Therefore, under this programme, a fibre optic probe-based He-Ne laser irradiation system to irradiate tissue in vivo and in vitro is being developed and standardized to ensure proper delivery of laser to the lesion, using mouse as a model. The tissue regeneration potential will be assessed using biochemical parameters such as estimation of collagen, hexosamine, hyaluronic acid and measurement of hsp-70 using standard protocols. Morphometric, histopathological and biochemical analysis of the biopsy from lesions will be performed at different stages of tissue regeneration. The proposed investigation will create low-level laser therapy facility for its application in enhanced tissue regeneration to be useful in clinical applications upon validation.